The correct collection sequence is: blood culture tube or bottle. Sodium citrate tubes (e.g. blue cap) Serum tubes, including those with clot activator and gel (e.g.
|2||Bright blue||Sodium citrate|
|5||Light green||Lithium heparin|
|Example by type:||Plasma or serum|
|Preferred collection container:||Stat / Line Pull: 3ml Green / Yellow Tube (Plasma Separator) Routine Requests / External Samples: 3.5ml Gold Top Tube (Serum Separator)|
|Alternative collection container:||3 ml lithium heparin tubes with green / black cap|
The same sequence is used to transfer blood from a syringe to the evacuated tube as it was used for collection. Do not press the plunger of the syringe while filling the tubes. The tube aspiration draws sample from the syringe until the tube is full.
When withdrawing from adults or adolescents, the aerobic vial (blue cap) should always be inoculated first to prevent air from escaping from the syringe into the anaerobic vial. Dispose of the needle and syringe properly (for example, in a sharp garbage can).
Green sealing tube with sodium or lithium heparin: Used for plasma or whole blood tests. EDTA tube: Contains a lavender cap, a pink cap (for blood bank tests), a beige cap (for lead tests) and a royal blue cap with EDTA (for the determination of trace metals in blood or in plasma).
Capillary blood collection has been around for decades. Capillary blood is obtained by inserting a finger in adults and a heel in infants and young children. The sample is then collected with a pipette, placed on a beaker or piece of filter paper, or taken from the tip of a micro-sampler.
63 cards in this set
When the capillary blood gases are controlled, they are first aspirated to minimize the entry of air into the environment. A blood count is done in front of the tubes with other anticoagulants and additives.
Light green tube (lithium heparin)
EDTA stands for ethylenediaminetetraacetic acid. EDTA works by binding calcium in the blood and preventing blood clotting. BD Vacutainer Plus Blood Collection Tubes contain K2EDTA, which is spray dried on the tube walls.
Any successful blood collection procedure involves a number of important steps:
To avoid cross-contamination of additives between tubes, blood must be drawn in a specific order. The procedure is the same for all types of tubes or tools used. It is important to follow the order not only to avoid contamination, but also to ensure the accuracy of the test results.
Sodium fluoride is used with other blood thinners, it is often added to help maintain blood sugar levels. It works by blocking an enzyme called enolase, which inhibits glycolysis. Potassium oxalate is an anticoagulant, it prevents the formation of blood clots by precipitating calcium.
All tubes (except red cap tubes which do not contain additives) should be gently inverted 5-8 times immediately after filling to ensure proper mixing of blood and anticoagulants or other additives.